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KMID : 1161420120150010066
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Journal of Medicinal Food
2012 Volume.15 No. 1 p.66 ~ p.70
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Cytotoxic, Trypanocidal, and Antifungal Activities of Eugenia jambolana L.
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dos Santos Karla K. A.
Matias Edinardo F. F.
Tintino Saulo R.
Souza Celestina E. S.
Braga Maria F. B. M.
Guedes Glaucia M. M.
Rolon Miriam
Vega Celeste
de Arias Antonieta Rojas
Costa Jose G. M.
Menezes Irwin A.
Coutinho Henrique D. M.
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Abstract
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Chagas' disease, caused by Trypanosoma cruzi, is considered a public health problem. Nowadays, chemotherapy is the only available treatment for this disease, and the drugs currently used, nifurtimox and benzonidazole, present high toxicity levels. Alternatives for replacing these drugs are natural extracts from Eugenia jambolana, a plant used in traditional medicine because of its antimicrobial and biological activities. An ethanol extract from E. jambolana was prepared. To research in vitro anti-epimastigote activity, T. cruzi CL-B5 clone was used. Epimastigotes were inoculated at a concentration of 1×105/mL in 200?μL of tryptose-liver infusion. For the cytotoxicity assay J774 macrophages were used. To examine antifungal activity, Candida albicans, Candida tropicalis, and Candida krusei were used. This is the first record of trypanocide activity for E. jambolana. The effective concentration capable of killing 50% of the parasites was 56.42?μg/mL. The minimum inhibitory concentration was ≤1,024?μg/mL. Metronidazole showed a potentiation of its antifungal effect when combined with the ethanol extract of E. jambolana. Thus our results indicate that E. jambolana could be a source of plant-derived natural products with anti-epimastigote and antifungal modifying activity with moderate toxicity.
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KEYWORD
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antifungal activity, anti-epimastigote activity, Chagas' disease, cytotoxicity, Eugenia jambolana, modifying activity
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